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ROS are counterbalanced by an antioxidant defence network, which functions to modulate ROS levels to allow their physiological roles whilst minimizing the oxidative damage they cause that can contribute to disease development

N-acetylcysteine targets 5 lipoxygenase-derived, toxic lipids and can synergize with prostaglandin E2 to inhibit ferroptosis and improve outcomes following hemorrhagic stroke in mice

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NaCl, ammonium sulphate, agar, acetonitrile, Tris-HCl, tetramethylethylenediamine, ammonium acetate and RNase were from Sigma-Aldrich (Missouri, USA)

These results highlight the trade-offs in developing screening assays and point to the benefit of screening with two assays