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Isolation and culture of primary proximal tubule epithelial cells (PTECs) Fresh mouse renal cortex was minced on ice and digested in HBSS with 0.75 mg/mL trypsin inhibitor and 0.75 mg/mL collagenase at 37 C for 20 min

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doi: 10.1148/radiology.213.2.r99nv42545 33 DesaiSRWellsAUSuntharalingamGRubensMBEvansTWHansellDM

These aggressors cause whats known as cellular destabilization, which leads to signs of aging, like sagging skin, fine lines, rough texture, and hyperpigmentation