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The oil red O stock solution was freshly mixed with Milli-Q water at a ratio of 3:2 by vortexing and filtrated using a 0.45 m filter

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To validate that tau seeding occurred in the MEA cultures, we collected the cells after the final recording and performed sELISAs for total tau (Tau5 antibody), PAD-exposed tau (TNT1 antibody), and oligomeric tau (TOC1 antibody) (Supplementary Fig

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Kurdekar et al., 2016)