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Similarly, it has been shown that colitis symptoms (colon shortening, DAI, loss of appetite, and weight gain), decreased SOD, and increased MPO can be mitigated and that GPx and GR activities ( Supplementary Table 1 ) can be increased by administering grape pomace extract (GPE) (204) decreased MDA, increased SOD, CAT, GPx, and GRx, and reduced iNOS and COX-2 gene expression in murine colitis

Fluorescence images were captured with a Zeiss Imager Z.1 fluorescence microscope (Zeiss) at 200x and 630 magnifications

coli MG1655 was transformed with plasmids directing the expression of Ssp4, either retained in the cytoplasm or fused to an N-terminal signal peptide for export to the periplasm (sp-Ssp4)

The knockdown of Caspase-1 could decrease the protein level of GSDMD and increase the ubiquitination levels of GSDMD but did not affect GSDMD mRNA, indicating that Caspase-1 might not directly regulate GSDMD

Curr Obes Rep 14 , 19 (2025)