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Differential metabolic pathways in LC patients versus the R and HC groups To obtain further insight into the differential metabolic characteristics of LC from R and HCs, we performed a metabolic pathway enrichment analysis ( ( Figure 3A )

Peptides were injected onto a 75 m 2 cm trap column (Thermo Fisher) before re-focusing on an Aurora Elite 75 m 15 cm C18 column (IonOpticks) using a Vanquish Neo UHPLC (Thermo Fisher) connected to an Orbitrap Astral mass spectrometer (Thermo Fisher)