Mechanistically, they demonstrated that STUB1 promoted polyubiquitination of GPX4 on lysine 191, leading to its degradation by the ubiquitin proteasome pathway
The assay was performed as described by 2.13 Proximity ligation assay (PLA) PC12 cells after fixation were blocked in the Duolink blocking solution in a humidity chamber for 30 min at 37C and incubated with the following primary antibodies: rabbit polyclonal anti-MVI and mouse monoclonal antibodies anti-B23, anti-fibrillarin, anti-UBF, anti-Pol1, anti-p-S6, and anti-S6, diluted in Duolink Antibody diluent solution for 3 h at 37C
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[125] found that chronic intake of a 60% fat diet in mice led to changes in the dopaminergic pathways
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