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The reaction mixture was centrifuged in a 3 kDa ultrafiltration tube (12,000 g , 4 min) and washed three times with PBS

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Furthermore, therapeutic drugs targeting Th17/Treg balance, including conventional and alternative therapies currently used in clinical practice, as well as emerging drugs under investigation, are reviewed

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Phenazopyridine used for urinary tract pain contains an azo dye, which gives it a reddish-brown color