hemochromatosis and glutathione Hemochromatosis—How Not to Overlook Properly Manage “Iron People”—A Review Iron Overload, Oxidative Stress, and

sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml

PMID 6822532

Altered serotonergic signaling is considered a hallmark neurochemical feature of anxiety disorders (Yohn et al., 2017)

GSTM22 has been shown to catalyze the conjugation of GSH to aminochrome, a ROS generated in the redox cycling of orthoquinones within dopaminergic neurons [60]

Risk factors for hereditary hemochromatosis include the presence of two copies of the abnormal gene, a positive family history, such as a parent or a sibling with the disease, being descended from a family of North European origin rather than of African, Hispanic or Asian origin