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Estimate your BAC and how long it may take for your BAC to return to 0.00

The 25-l reaction solution contained 6 g of nuclear extract , 5 l of 5 binding buffer (50 mM Tris-HCl, pH 7.4, with 20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 5 mM DTT, and 0.25 mg/ml poly dI-dC) and 50,000 cpm labeled probe