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Throughout the process of pyroptosis, caspase 1 is responsible for cell swelling (Fink and Cookson, 2006), DNA fragmentation (Bergsbaken and Cookson, 2007), the arrest of cell metabolism (Shao et al., 2007), and other related functions (Figure 3)

[6] [2] [3] Severe vitamin B 12 deficiency can damage nerve cells

The terminal hydroxyl group forms hydrogen bonds with the side chains of Gln72 and Gln164, and its replacement with an amine would significantly weaken the binding affinity

Consequently, the bacterias ability to maintain metabolic and homeostatic functions becomes compromised, aligning with previous findings indicating increased susceptibility to additional oxidative stress ( 5 Conclusion Our study highlights the profound metabolic perturbations induced by -lactam antibiotics in E.coli , elucidating distinct yet overlapping effects across different antibiotic classes