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CD47 the surface protein CD47 is a receptor that binds to signal-regulatory protein alpha (SIRP) or thrombospondin-1 (TSP-1) and acts as a signal to inactivate the phagocytosis in macrophages

Jan 15 2021;28(5):529-537

Oxygen consumption was monitored for 80 s and then 0.2 mM ADP was added to stimulate state three respiration

After acetone precipitation, the resulting protein pellet was resuspended in DIGE rehydration buffer (DRB) (7 M urea, 2 M thiourea, 4% wt/vol CHAPS, Tris 20 mM, pH 8.5)

Assessment of risk of bias The risk assessment is conducted according to Cochrane renal group, and the selection bias is evaluated by random sequence generation and promotion, the performance bias is evaluated by blinding of investigators and participants, the detection bias is evaluated by blinding of outcome assessors, the attrition bias is evaluated by incomplete outcome data, the reporting bias is evaluated by selective reporting and possibly other sources of bias