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The fold changes of aspartate, glutamate, asparagine, and glutamine were calculated based on their relative abundances in MSO-treated and untreated controls or Pb GSKO and Pb WT samples, after normalizing with the average fold changes of the relative abundancies obtained for arginine, serine, histidine, threonine and tyrosine

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If you discontinue treatment without maintaining those changes, weight regain is possible