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Likewise, ESX-3 and ESX-5 also facilitate pathogenesis through the secretion of Esx proteins as well as members of the proline-glutamic acid (PE) and proline-proline-glutamic acid (PPE) protein family (characterised by their Proline and Glutamic acid N-terminal motifs) [5,6,7]

Med Sci Monit (2012) 18(4):BR1304

Membranous nephropathy associated with immunoglobulin G4-related disease successfully treated with obinutuzumab

2.12 Colony-formation analysis Cells (1000 per well) were plated in culture plates for 1 weeks at 37C in a humidified environment with 5% CO 2 and stained with crystal violet staining solution(1%) for 10~20 min

Morning works well if you eat a fat-containing breakfast, while evening may be better if dinner is your largest meal