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10.1007/s10803-021-04928-7 J

Aged green tea reduces high-fat diet-induced fat accumulation and inflammation via activating the AMP-activated protein kinase signaling pathway

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SDS-page, and semiquantitative immunoblotting Amount of proteins was equalized among samples and disulfide bonds were reduced by the addition of Laemmli sample buffer and heating to 95 C for 5 min prior to loading on any-kD polyacrylamide gels (Bio-Rad, 4561086)