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1:700), NRF2 antibody (Cell Signaling, cat:336493, 1:2000), GPX4 antibody (Abcam, cat:ab125066, 1:2000), SLC7A11 antibody (Abcam, cat:ab175186, 1:2000), Rab27a antibody (Zen-Bioscience, cat:340884, 1:1000), -actin antibody (Cell Signaling, cat:3700, 1:2000)

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Pyruvate prevents the onset of the cachectic features and metabolic alterations in myotubes downregulating STAT3 signaling

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More information on the sample preparation is provided in the Supplementary information

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