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doi:10.1007/s12020-016-1098-z Zheng H, Wei J, Wang L, et al

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Thereafter, lungs were either frozen to isolate RNA, inflation-fixed with paraformaldehyde for histology or lavaged to collect bronchoalveolar lavage fluid ( RNA ANALYSIS Total RNA was isolated from frozen lung tissue, quantified by spectrophotometry and assayed for messenger RNA transcript abundance using standard real time PCR methodology as described ( HISTOLOGY AND CYTOLOGY Standard techniques were used to fix lung tissue in 4% paraformaldehyde and to embed in paraffin

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Meanwhile, in RAAS, ACE's conversion of angiotensin I to angiotensin II, which has a potent vasoconstrictor impact, may cause the adrenal cortex to generate more aldosterone, both of which raise blood pressure