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10.1038/s41419-018-0469-1 128 LiuT.ZongS.LuoP.QuY.WenY.DuP.et al (2019)

Exogenous melatonin supplementation prevents oxidative stress-evoked DNA damage in human spermatozoa

These molecular modifications alleviate the rheological difficulties of circulation in hypoxic conditions and diminish the probability of premature erythrocyte clearance

Prior to image cytometry, cells were incubated in FACS buffer at 37C with 100 g/ml RNase A for 2 h, washed once with 1 ml of PBS, and resuspended in 1 ml of PBS