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The results confirmed the presence of a strong electrophilic centre at the tertiary carbon for both FCCP and NMY1009, which reacts with nucleophilic GSH

Thereafter, the cells were incubated with primary mouse monoclonal antibody to H2AX (Ser-139) diluted 1 : 1000 (Upstate Biotechnology) or primary antibody anti-cisplatin (CP9/19) (Abcam, Cambridge, MA, USA) that was diluted 1 : 5000 for 16 h at 4 C

These assays included bacterial mutagenicity tests with Salmonella and E

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PMCID: PMC7589152 Tian R, Yang Y, Chen M (2020) Genome-wide survey of the amino acid transporter gene family in wheat ( Triticum aestivum L.): identification, expression analysis and response to abiotic stress