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Finally, the fluorescent cells were washed three times with 2 mL of phosphate-buffered saline (pH 7.4) for 10 min at 2000 g in a centrifuge

Gastroenterology 116 :874884

Conclusion and perspectives Excessive accumulation of glutamate not only leads to excitotoxicity, but also to ferroptosis, Therefore, maintaining glutamate homeostasis is essential to inhibit excitotoxicity and ferroptosis