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After collecting the 72-h samples, we allowed the MEFs from three independent embryos to continue reprogramming up to 10 days, when iPS cells can be seen by light microscopy and stain positive for alkaline phosphatase

To test binding of z13 peptide to CNGB3MYC, FITC-z13 was added (5 g ml 1 ) to the medium of HeLa cells transfected with the above-described vector and incubated at 37 C for 15 min

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Early prevention is also important, and active screening of high-risk individuals and initiation of treatment prenatally may be effective in preventing brain malformations in affected children

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