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L1-Red/ L1-DiS/ L1-CCA input phage (~ 10 11 ) and streptavidin-coated magnetic Dynabeads (50 L/ selection, prewashed with 1 mL PBS) were incubated with blocking buffer (volume made up to 500 L for phage, 1 mL for beads) for 1 h on a rotary mixer at room temperature and 20 RPM

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The existence of the trans-sulfuration pathway (TSP) in mammals indicates that in case of dietary cysteine scarcity, the host shuttles homocysteine from the methionine cycle via the production of cystathionine, which is then hydrolysed into cysteine by the enzyme cystathionine -lyase (CTH) 10,13

Another pathway implicated in neuroinflammation is janus kinase-2 (JAK-2)/signal transducer and transcription-3 (STAT-3) cascade that is activated by the effect of pro-inflammatory mediators