CAT: catalase DHODH: Dihydroorotate dehydrogenase FSP1: Ferroptosis suppressor protein 1 TEM: Transmission electron microscopy AMPK: Adenosine monophosphate-activated protein kinase M-TOR: Mammalian target of rapamycin PCa: Prostate cancer References Kim EH, Larson JA, Andriole GL
Dead volume and actual yield Every time you insert a needle into a vial and draw solution, a small amount of liquid remains in the needle hub, in the rubber stopper, and coating the glass walls
Plus, it might be helpful to note that you do not have the needle in place for the entirety of the treatment session
doi: 10.1016/j.bbadis.2011.12.009 75 FreeseC.HanadaS.Fallier-BeckerP.KirkpatrickC
Basic Calculation Framework To determine how much peptide is delivered per spray, use: Concentration (mcg/mL): Final solution strength after reconstitution (Convert if needed: mg/mL 1000 = mcg/mL) Spray Output (mL per actuation): Volume delivered per pump Formula mcg per spray = concentration spray volume Optional Unit-Based Conversion (U-100 Reference) If using insulin-style units as a volume reference: 1 mL = 100 units Spray volume (mL) = units 100 Updated Formula mcg per spray = concentration (units 100) Calculation Table (Template) Worked Examples (For Understanding Only) Example 1 (mL-based device): Concentration: 3333 mcg/mL (10 mg in 3 mL) Spray output: 0.10 mL mcg per spray = 3333 0.10 = 333.3 mcg Sprays per mL = 10 Total sprays (3 mL) = 30 sprays Example 2 (unit-based device): Concentration: 3333 mcg/mL Device output: 13 units Volume per spray = 13 100 = 0.13 mL mcg per spray = 3333 0.13 = 433.3 mcg Sprays per mL 7.7 Total sprays (3 mL) 23 sprays Final Note These examples are based on a 10 mg / 3 mL reconstitution for demonstration only
