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Description

For the SPRDtgHD rat tissues, 8-month-old animals were killed via transcardial perfusion with perfusion buffer (0.01 M PBS, pH 7.4)

Histopathology of Colon Tissues The colon tissues of rats were removed, fixed with 4% paraformaldehyde in PBS, and then decalcified for 10 days with ethylenediaminetetraacetic acid and embedded in paraffin for histological analysis