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Immunoblot and quantification of indicated proteins in primary hepatocytes isolated from mice liver treated with BSA, 200 M palmitic acid (PA), oleic acid (OA), or linoleic acid (LA) for 4 h after overnight serum starvation, and immunoblot quantification of P-eIF4E/eIF4E in the AML12 cells and primary hepatocytes in response to BSA or different fatty acids (n = 3 biological independent samples)

Andrews PW, Bharwani A, Lee KR, Fox M, Thomson JA

Initial column temperature was 90 C held for 1.5 min, increased to 220 C at a rate of 20 C min 1 , followed by a second ramp to 320 C at a rate of 10 C min 1 and held for 10 min

The two methods of Viticis Fructus recorded in Boji Fang were washed Viticis Fructus, baked with mild fire then crushed (Wang, 1958a)

According to IRAC [34] OTA is classified as group 1 carcinogens