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Autophagy's connection to aging and age-related disease lies in a couple of factors

To set up binding reactions, the mixtures of 20 nM FAMBRD BD1 and 100 nM YPEL5WDR26 CTLH or WDR26 CTLH in the binding buffer (25 mM HEPES pH 7.5, 150 NaCl, 5 mM DTT, 1 mg ml 1 BSA and 0.1% Tween-20) were combined with equal volumes of a twofold dilution series of the degraders or JQ1 (prepared in DMSO and then diluted with the binding buffer to reach the final DMSO concentration of 2% for all titration points) and incubated for 1 h at room temperature before transferring to 384-well flat-bottom black plates (Greiner)

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