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Aim for 810 seconds to deliver a 0.3mL dose

Previous studies on CUR have focused primarily on its applications in poultry [4, 23, 25] and rodents [20, 31, 32]

Both the patient and their family should be emotionally prepared for the treatment process

Confocal immunofluorescence microscopy All cells were fixed for 15 min with 4% cold paraformaldehyde, permeabilized with 0.2% Triton X-100, and stained for immunofluorescence microscopy