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doi: 10.1369/0022155413475452 41 QinYHuQXuJJiSDaiWLiuWet al

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Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.05% Tween 20 for 1 h at room temperature before overnight incubation at 4C with a 1:1,000 dilution of primary antibodies against total and phosphorylated AMPK and total and phosphorylated ACC (cat

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Biosci Biotechnol Biochem 80:540546