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Though short term (48 hours) resistance to MTX seemed to be directly related to cellular proliferation, resistance in longer exposure times (96 hours) was associated with glutathione (GSH) levels

Contribution to oxidative stress and interorganellar signaling

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While the pathological and clinical presentations of neurodegenerative diseases vary, they share common features including specific pathological alterations within distinct brain regions and the degeneration of various neuronal subtypes

231,232 These modifications alter gene expression and protein function, causing vascular dysfunction, inflammation, and oxidative stress, all of which contribute to the pathogenesis of DPN