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Description

Analytical challenges in multi-residue citrus fruit extraction arise from individual matrix characteristics

Eur Heart J Open 2024;4:oeae035

Egan, et al

PCR products of candidate clones were further cloned into the pRK5 vector, followed by sequencing to confirm the knockout of RSMC at the single-allele level

Together these results suggest that ORF3a induces mROS production to activate HIF-1, which in turn triggers a shift in cellular metabolism to favor glycolysis, resulting in increased viral replication and transcription of pro-inflammatory cytokines