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4) The compound of target protein and GST-tagged protein Transfer the target Protein to the Glutathione Magarose Beads binding with GST-tagged protein

Result: In the presence of up to 5 mM TCEP, 10 mM DTT, or 50 mM -ME, the amount of His-tag protein being purified from Ni-Penta resin was similar to those being purified in absence of the respective reducing agents

doi: 10.1007/s00011-020-01401-6 23 IbrahimH.PerlA.SmithD.LewisT.KonZ.GoldenbergR.et al

[DOI] [PubMed] [Google Scholar] 105.Eren E., Tufekci K.U., Isci K.B., Tastan B., Genc K., Genc S

Research from the University of Miami Miller School of Medicine has shown that specific nutrients including carnitine, CoQ10, and B vitamins play crucial roles in mitochondrial energy production