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Protein isolation and western blotting For standard western blotting, tissues were lysed in RIPA buffer and extracted protein content was determined using the BCA protein assay (Sigma, St

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The essential nature of GPX4 is demonstrated by the fact that genetic disruption of the entire Gpx4 gene in homozygous Gpx4 knockout causes in utero fetal death by midgestation (Imai et al., 2003)