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Unlike CRBN, which has played a central role in the development of targeted protein degradationand proximity pharmacology, more broadlyFBXO3 has not been rewired by CIPs previously, at least to our knowledge

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Then, endogenous peroxidase activity was quenched using a solution of 3% H 2 O 2 , and 10% methanol in PBS for 30 minutes

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To keep the cycle going, another enzyme called glutathione reductase (GR) uses NADPH to convert GSSG back to GSH, creating a sustainable loop of protection