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Nuclear gene therapy represents an alternative strategy, as numerous mitochondrial proteins are encoded by nuclear genes

Additional factors have been implicated in the induction of ferroptosis 13 , including the engagement of mitochondrial metabolism and the tricarboxylic acid (TCA) cycle 14,15

Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR, 0777), and incubated overnight with the following primary antibodies: ACC (1:1000