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The cell suspension (100 l) was incubated with Annexin V-FITC (5 l) and propidium iodide (PI) (5 l) for 15 min at room temperature

115 Hyperglycemia induces vascular calcification via AGE accumulation, leading to hydroxyapatite deposits in both the intimal and medial layers, characteristic of atherosclerotic plaques and medial arterial calcification

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