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Because of this difference a concentration gradient amenable to flow exists and the Na+/K+ ATPase pump effects the transfer of these two ions pushing out 3 Na+ ions for every 2 K+ ions pumped back into the cell

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According to Herculano-Houzel, 2014)

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Due to the inability of sucrose molecules to traverse cell membranes, the modified HTK-N solution utilizes sucrose in place of mannitol within the HTK solution (111)