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RNA Extraction, Reverse Transcription, and Quantitative Real-Time PCR In order to examine the qRT-PCR results, RNAiso-mate Tissue Kit was used to isolate total RNA from frozen fruit tissue (Tiangen, Beijing, China)

doi: 10.2174/138945010791591395 20 XueMJacksonCJ

The methodology of this study includes establishing an LL37-induced rosacea-like mouse model and observing the therapeutic effects of either combined treatment with minocycline (MIN) and hydroxychloroquine (HCQ) or GBP treatment alone, with the combined MIN and HCQ treatment serving as the positive control

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A two-stage exacerbated hypoxia nanoengineering strategy induced amplifying activation of tirapazamine for microwave hyperthermia-chemotherapy of breast cancer