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Which translates to 0.028mL or roughly 3 units on an insulin syringe at 250mcg/0.1mL concentration

The mislocalization of Pf GS was induced by the addition of 250 nM rapamycin (Sigma-Aldrich, R0395) and verified by the translocation of GFP fluorescence from the parasite cytosol to the plasma membrane in live parasites

One tool worth exploring involves metaphor, discussed under the Therapeutic Disclosure section that follows

and Cao, J

The l-EV fraction from FLAG-MIM I-BAR- and Halo7-Rac1-co-expressing cells was serially diluted in the lysis buffer, and the Rac1 amount was measured by ELISA simultaneously with the cell lysates