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In: McManusLMMitchellRN, editors

Selective suppression of hippocampal ripples impairs spatial memory

(B) Functionality of the dCas9-based transcriptional repressor is demonstrated by a 5.7-fold decrease in CD81 protein expression (red histogram) compared to non-targeting gRNA controls (grey histogram), measured by geometric mean fluorescence intensity (GMFI) in the GFP+ population

58,59 As cellular cysteine availability is the rate-limiting step in glutathione synthesis, 59 the function of the xCT transporter, and therefore intracellular glutamate concentrations, are critical for the synthesis of this major cellular antioxidant