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For PICS profiling, 25 g of peptide library was incubated at 37 C with 0.5 g of enzyme in 50 L of either 100 mM glycine pH 2.5, 150 mM sodium chloride (active Clc due to acidic pH

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[16] [2] It is important to check and replace folate after starting B12 therapy, as coexisting deficiencies are common and correcting B12 alone can precipitate or worsen folate deficiency