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As the period of clinical manifestation of the disease generally occurs on average 20 to 38 days after the calf has contact with the pathogen, blood was collected on a fixed date of 20 days after inoculation (60 days of experiment) to maintain equal inoculation days among animals

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Primarily, the available research is often characterized by small study populations and short intervention durations, which limit statistical power and the ability to fully understand long-term effects

Mechanism underlying increased neuronal activity in the rat ventrolateral periaqueductal grey by a mu-opioid