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Luger et al

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This approach makes intuitive sense because the amount of incretin stimulation needed to maintain a lower weight is probably less than the amount needed to achieve that lower weight in the first place

Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added