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Nevertheless, methotrexate has considerable adverse effects, particularly hepatotoxicity, which necessitates regular monitoring of liver function and adaptations to the dosage based on patient tolerance (Barnhill et al

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Disruptions to these processes or abnormalities in the participating enzymes can silence tumor suppressor genes [26, 27]

Microbiota-based interventions have been shown to induce behavioral changes in ASD animal models, with metabolic modulation emerging as a primary pathway through which the gut microbiota exerts its influence on the CNS