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JAMA Netw Open 3(7):e2011034 2020 Robertson CC, Inshaw JRJ, Onengut-Gumuscu S, Chen WM, Santa Cruz DF, Yang H, Cutler AJ, Crouch DJM, Farber E, Bridges SL, Jr., Edberg JC, Kimberly RP, Buckner JH, Deloukas P, Divers J, Dabelea D, Lawrence JM, Marcovina S, Shah AS, Greenbaum CJ, Atkinson MA, Gregersen PK, Oksenberg JR, Pociot F, Rewers MJ, Steck AK, Dunger DB, Type 1 Diabetes Genetics Consortium, Wicker LS, Concannon P, Todd JA, Rich SS

Ortolani F, Piccinno E, Grasso V, Papadia F, Panzeca R, Cortese C, Felappi B, Tummolo A, Vendemiale M, Barbetti F (2016) Diabetes associated with dominant insulin gene mutations: outcome of 24-month, sensor-augmented insulin pump treatment

Such measures help support internal quality procedures and strengthen procurement confidence

Co-immunoprecipitations To confirm the physical interaction between DDX3X and pU L 31, HeLa cells were grown on 100 mm dishes, mock-treated or infected with HA-tagged U L 31 virus (vJB90) or wild-type strain 17 + at an MOI of 5 for 12 h

On the following day, the dialysed eluate was purified by anion-exchange chromatography on either a Resource Q column (Cytiva, 17-1179-01) or a HiTrap Q XL column (Cytiva, 17515901) pre-equilibrated in buffer A (50 mM Tris pH 8.0, 100 mM NaCl, 5% (v/v) glycerol and 4 mM BME)