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Epilepsia 47 , 421424 (2006)

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To lessen the particle size, liposomes were exposed to sonication utilizing a probe sonicator [50] (Branson SFX150, Branson, USA) at 50% amplitude for 10 mins (Pulse 45 s and Stop 45 s)

This solution containing 27.6% (w/v) Nycodenz in 3 mM KCl, 0.3 mM CaNa2-EDTA 5 mM Tris-HCl, pH 7.5 (density = 1.15 g/ml) was subsequently diluted with the same KCl, EDTA, Tris solution containing 0.75 g NaCl to produce solutions of 18.4% and 13.8% Nycodenz ( = 1.098 and 1.075 respectively)