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Wnt ligand-bound seven-pass transmembrane receptor complex consists mainly of the Frizzled (Fzd) family proteins and its co-receptor complexes, including low-density lipoprotein receptor-related proteins (LRP5/6) (72)

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coli BL21(DE3) cys cells cotransformed with plasmids pCGPx3M and pMazF (TaKaRa) were grown at 37C in 400 mL of MM medium (modified M9 minimal medium) supplemented with ampicillin (100 g/mL), kanamycin (25 g/mL), chloramphenicol (25 g/mL) and Cys (50 g/mL) on a shaker

The initial conjugates are then transformed sequentially to ITC-cysteinylglycine (ITC-CysGly), ITC-cysteine (ITC-Cys), and ITC- N -acetylcysteine conjugates (ITC-NAC) through the mercapturic acid pathway that predominates in mammals and in most generalist herbivores, including the Egyptian cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae) 24,26,27