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doi: 10.13344/j.microbiol.China.230433 48 JinM.ShiC.LiT.WuY.HuC.HuangG

Beyond sequencing: machine learning algorithms extract biology hidden in Nanopore signal data

After destaining, the membrane was blocked using 5% BSA in Tris buffered saline with Tween 20 (TBS-T) (Catalogue #T9039, Millipore Sigma, Burlington, MA, USA) for 1 h at room temperature followed by incubation with a polyclonal Donkey anti-mouse IgG antibody (Catalogue# 926-68072, LICORbio, Lincoln, NE, USA) ( Supplementary Table 1 ) diluted 1:2000 in blocking buffer for 1 h at room temperature followed by three washes for 5 min each with TBS-T

One basic feature is enhancing the drug delivery across the BBB, which ensures that a higher concentration of the therapeutic agent reaches the brain (122)