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Following this, cells were washed with permeabilization buffer (Biolegend) and then stained with intracellular antibody cocktail ( Table 1 ) for 30 mins on ice, wash with Biolegend permeabilization buffer then stained with metal-conjugated streptavidin for 10 mins on ice

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The enzyme activity site must have ATP/ADP bound for the enzyme to be active, while the substrate specificity site will bind different nucleotides influencing the enzyme substrate preference, therefore altering which base is being acted upon depending on cellular needs

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