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Commercially available crotonic acid was used as a standard in parallel with a conversion ratio of 0.893 (Koch et al., Non-targeted Metabolome Profiling Analysis by Gas Chromatography-Mass Spectrometry (GC-MS) The metabolome profiling of the metabolite fraction enriched with primary metabolites was performed as previously described (Kopka et al., 750 = 0.71.0 cultures were extracted for 15 min at 37C by 1 ml methanol:chloroform:diethylamine 2.5:1.0:1.0 (v/v/v)

1 Introduction Periodontitis is a globally prevalent chronic inflammatory disease triggered by dysregulation of oral biofilm and host immune responses, serving as the primary cause of adult tooth loss (1)

Intramuscular (IM) injections go deeper, directly into the muscle tissue

Apply a cotton ball without rubbing