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The DNA binding reactions with RPA were carried out in the pulldown buffer (20 mM HEPES pH 7.5, 5% glycerol, 200 mM NaCl, 1 mM TCEP, 0.1% Tween-20) containing either fluorescently labelled ssDNA (50 nM, oWL985) or D-loop substrate (50 nM)

Acknowledgements This work was supported, in part, by National Institutes of Health (NIH) grants R01NS094533 (to Y.F.), R01NS106108 (to Y.F.), R01CA241501 (to Y.F.) and R01HL155198 (to Y.F.), and American Cancer Society Yosemite Award 24-1331395 (to Y.F.)

doi: 10.1128/mmbr.59.3.506-531.1995 55 HambrookJRKaborALPilaEAHaningtonPC

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Argast, M