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Description

Immunoprecipitation (IP) and Co-immunoprecipitation (Co-IP) assay Cells or tissues were lysed in IP lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.5% Triton X-100, 0.5% NP-40, 1 mM EDTA, and 1 mM EGTA, 1 mM -glycerophosphate, 1 mM PMSF, and 1X PIC)

Hober S, Ljung JL, Uhln M, Nilsson B (1999) Insulin-like growth factors I and II are unable to form and maintain their native disulfides under in vivo redox conditions